VALIDAtIoN oF tLc – DeNsItoMetRIc MetHoD FoR IDeNtIFIcAtIoN AND DeteRMINAtIoN oF estRADIoL

The aim of current study was the validation of TLC densitometric method for identification and determination of Estradiol hemihydrate in dosage forms. The TLC conditions were: glass plates with Silicagel G60F254; mobile phase: chloroform : water = 90 : 10 v/v. Linearity accordance between the concentration and spot area in range: 5.10-4 g/ml ÷ 5.10-3 g/ml is proved by the regression equation: y = 53256970.x – 7007. The results of the recovery studies presented in R [%] ± RSD [%] suit respective confidence interval: 1) RС1.5: 98.75 % ÷ 102.13 % (SD = 1.01); 2) RС2: 98.73 % ÷ 102.93 % (SD = 1.25); 3) RС2.5: 98.29 % ÷ 103.03 % (SD = 1.4). All data for the obtained quantity of Estradiol hemihydrate correspond to the confidence interval: 1.95 mg ÷ 2.09 mg (SD = 0.05). The proposed validated TLC densitometric method is appropriate for quality control of Estradiol hemihydrate in commercially available tablets.